The presence of genotoxic substances in the river Danube five months after bombardment of the industrial zone of Pančevo as revealed by the Allium anaphase-telophase genotoxicity assay

The consequence of aerial bombardment of the petrochemical complex, oil refinery and chemical industry including the HIP 'Azotara' fertilizer factory in the vicinity of Pančevo in April 1999 was that a large amount of dangerous chemicals leaked into the canal collecting industrial wastewater and flowed pouring out into the Danube. As the level of water in the Danube was high at that time, leaking of chemicals from the canal into the river lasted for a long time. Therefore the aim of this work was to check if toxic and genotoxic effects were present 5 months after the bombardment. Three samples of Danube water (upstream from the canal, just after the opening of the canal and downstream from the canal) were used for this purpose. All three samples plus a sample from the canal were also chemically analyzed. The Allium anaphase-telophase test was used to examine the toxicity and genotoxicity of water samples because it can be used without any concentration or purification of samples before testing. Clear inhibition of growth, compared to the control sample, was produced by the second sample. This sample, taken just after entry of the canal was the only one which produced a statistically significant increase in the number of chromosome aberrations in comparison with the control sample (X2(i) = 10.7, p<0.001). The results of our test and the chemical analyses of the water samples, show that both toxic and genotoxic effects of the Danube water may occur due to water coming in from the canal even 5 months after the chemicals had leaked into the canal.Kao posledica bombardovanja Petrohemijskog kompleksa, Rafinerije nafte i hemijske industrije Pančevo - HIP Azotara u blizini Pančeva u aprilu 1999. godine izlile su se velike količine opasnih hemikalija u kanal za industrijsku otpadnu vodu, a njime u Dunav. Pošto je u to vreme nivo vode Dunava bio visok izlivanje hemikalija u reku trajalo je dugo vremena, tako da je cilj našeg rada bio da se proveri da li su toksični i genotoksični efekti prisutni 5 meseci nakon bombardovanja. Za tu svrhu analizirana su tri uzorka Dunavske vode (uzvodno od kanala, na ušću kanala i nizvodno od kanala). Svatri uzorka kao i uzorak vode iz kanala hemijski su analizirani. Za ispitivanje toksičnosti i genotoksičnosti uzoraka vode korišćen je Allium anaphase-telophase test zbog činjenice da se uzorci mogu ispitivati bez koncentrisanja ili prečišćavanja. Drugi uzorak pokazao je jasnu inhibiciju rasta korenčića luka u odnosu na kontrolni uzorak (toksični efekat). Isti uzorak je bio jedini koji je produkovao statistički značajan porast broja hromozomskih aberacija u poređenju sa kontrolom (X2(i) = 10.7, p<0.001). Rezultati testiranja kao i rezultati hemijske analize pokazuju da su tokskični i genotoksični efekati u vodi Dunava poreklom iz vode koja dolazi iz kanala.nul

A Detailed XRD and FTIR Analysis of Bi2O3 Doped ZnO-SnO2 Ceramics

Poster presented at the 2nd International Congress on Ceramics, Verona, Italy, June 29 - July 4, 200

OUTAGE PERFORMANCE OF COGNITIVE RF/FSO SYSTEM WITH MRC SCHEME AT THE RECEIVER

The aim of this paper is performance analysis of the hybrid radio frequency (RF)/free-space optical (FSO) system, where the transmission is performed simultaneously over FSO link and spectrum sharing cognitive RF sub-system. The FSO link is affected by Gamma-Gamma atmospheric turbulence, while in spectrum sharing cognitive RF sub-system the peak interference power constraint at the primary user's receiver is considered in Nakagami-m fading environment. Outage probability expressions are provided in the integral form for the case when the maximal ratio combining (MRC) is applied at the destination. The effects of the atmospheric turbulence strength, the number of RF antennas, allowable power and fading severity on the outage performance are observed. Numerical results are presented and verified by Monte Carlo simulations

The presence of genotoxic substances in the river Danube five months after bombardment of the industrial zone of Pančevo as revealed by the Allium anaphase-telophase genotoxicity assay

The consequence of aerial bombardment of the petrochemical complex, oil refinery and chemical industry including the HIP 'Azotara' fertilizer factory in the vicinity of Pančevo in April 1999 was that a large amount of dangerous chemicals leaked into the canal collecting industrial wastewater and flowed pouring out into the Danube. As the level of water in the Danube was high at that time, leaking of chemicals from the canal into the river lasted for a long time. Therefore the aim of this work was to check if toxic and genotoxic effects were present 5 months after the bombardment. Three samples of Danube water (upstream from the canal, just after the opening of the canal and downstream from the canal) were used for this purpose. All three samples plus a sample from the canal were also chemically analyzed. The Allium anaphase-telophase test was used to examine the toxicity and genotoxicity of water samples because it can be used without any concentration or purification of samples before testing. Clear inhibition of growth, compared to the control sample, was produced by the second sample. This sample, taken just after entry of the canal was the only one which produced a statistically significant increase in the number of chromosome aberrations in comparison with the control sample (X2(i) = 10.7, p<0.001). The results of our test and the chemical analyses of the water samples, show that both toxic and genotoxic effects of the Danube water may occur due to water coming in from the canal even 5 months after the chemicals had leaked into the canal.Kao posledica bombardovanja Petrohemijskog kompleksa, Rafinerije nafte i hemijske industrije Pančevo - HIP Azotara u blizini Pančeva u aprilu 1999. godine izlile su se velike količine opasnih hemikalija u kanal za industrijsku otpadnu vodu, a njime u Dunav. Pošto je u to vreme nivo vode Dunava bio visok izlivanje hemikalija u reku trajalo je dugo vremena, tako da je cilj našeg rada bio da se proveri da li su toksični i genotoksični efekti prisutni 5 meseci nakon bombardovanja. Za tu svrhu analizirana su tri uzorka Dunavske vode (uzvodno od kanala, na ušću kanala i nizvodno od kanala). Svatri uzorka kao i uzorak vode iz kanala hemijski su analizirani. Za ispitivanje toksičnosti i genotoksičnosti uzoraka vode korišćen je Allium anaphase-telophase test zbog činjenice da se uzorci mogu ispitivati bez koncentrisanja ili prečišćavanja. Drugi uzorak pokazao je jasnu inhibiciju rasta korenčića luka u odnosu na kontrolni uzorak (toksični efekat). Isti uzorak je bio jedini koji je produkovao statistički značajan porast broja hromozomskih aberacija u poređenju sa kontrolom (X2(i) = 10.7, p<0.001). Rezultati testiranja kao i rezultati hemijske analize pokazuju da su tokskični i genotoksični efekati u vodi Dunava poreklom iz vode koja dolazi iz kanala.nul

Changes of Structural, Optical and Electrical Properties of Nickel-Manganite Ceramics Induced by Additional Mechanical Activation

Poster presented at the 10th International Conference and Exhibition of the European Ceramic Society, Berlin, June 17-21, 200

Far infrared properties of iron doped single crystal PbTe

Far infrared reflectivity spectra of single crystal PbTe samples doped with 0.2 at% Fe were measured in the temperature range between 10 K and 300 K. These spectra show very sharp and narrow dips below 120 cm-1. The experimental data were numerically analyzed using a fitting procedure based on the plasmon-phonon interaction model. Two local modes were observed. The first one was noted between 130 cm-1 and 170 cm-1 depending on the temperature and the second more frequency stable one was noted at about 230 cm-1

Optical far infrared properties of FeS2

In this paper are presented the far-infrared reflectivity spectra for a cleaved FeS 2 sample measured at roam temperature and at 77 K

Optical far infrared properties of PtSb2

Far infrared reflection spectra measured at room temperature were used to investigate vibrational properties of PtSb2 single crystals. The experimental results were analyzed using a dielectric function taking into account the existence of plasmonionised impurity-phonon interactions. Together with strong coupling three infrared active lattice modes at about 143, 187 and 202 cm-1 were observed. These results were discussed with respect to calculated literature vibrational frequencies. Electrical properties of single crystal PtSb2 were also measured at room temperature

Far infrared properties of PbTe doped with Hg

Single crystal samples of PbTe doped with Hg were grown using the Bridgman method. Far infrared reflectivity spectra were measured at room temperature for samples with 0.5 at. % Hg; 0.9 at. % Hg and 1.4 at. % Hg. The plasma frequency decreased when PbTe was doped with Hg and it was lowest for the PbTe sample doped with 0.5 at. % Hg. The values of the determined optical free carrier mobility increased and was the highest for PbTe doped with 0.5 at. % Hg

Supplementary data for the article: Brasanac-Vukanovic, S.; Mutic, J.; Stankovic, D. M.; Arsic, I.; Blagojevic, N.; Vukasinovic-Pesic, V.; Tadic, V. M. Wild Bilberry (Vaccinium Myrtillus l., Ericaceae) from Montenegro as a Source of Antioxidants for Use in the Production of Nutraceuticals. Molecules 2018, 23 (8). https://doi.org/10.3390/molecules23081864

Supplementary material: [https://doi.org/10.3390/molecules23081864]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2221